Journal: Cancers
Article Title: Paracrine IL-6 Signaling Confers Proliferation between Heterogeneous Inflammatory Breast Cancer Sub-Clones.
doi: 10.3390/cancers14092292
Figure Lengend Snippet: Figure 1. Localization of HER2, pSTAT3 and IL-6 in human inflammatory breast cancers. FFPE sections from human inflammatory breast cancer pre-treatment core biopsies were co-stained with HER2 (red), IL-6 (green) and pSTAT3 (white) antibodies using multiplexed multispectral imaging and counterstained with DAPI (blue) to visualize the nuclei (see Section 2). The specimen shown is a luminal A (ERpos, PgRpos, HER2neg) tumor (INFLAM #2, Table S1) which displays co-localization of cell surface HER2 expression and nuclear pSTAT3 in a cluster of tumor cells with diffuse IL-6 positivity in an adjacent tumor cell cluster. Scale bar represents 100 µm.
Article Snippet: The antibodies used were rabbit polyclonal anti-human IL-6 (Abcam, Melbourne, VIC, Australia); mouse monoclonal anti-human HER2, clone 3B5 (Merck Millipore); rabbit polyclonal anti-human pSTAT3 (Y705) (Cell Signaling Technology).
Techniques: Staining, Imaging, Expressing